Chromatin-remodeling protein that binds DNA through histones and regulates gene transcription.
May specifically recognize and bind trimethylated 'Lys-27' (H3K27me3) and non-methylated 'Lys-4' of histone H3.
Plays a role in the development of the nervous system by activating the expression of genes promoting neuron terminal differentiation.
In parallel, it may also positively regulate the trimethylation of histone H3 at 'Lys-27' thereby specifically repressing genes that promote the differentiation into non-neuronal cell lineages.
Tumor suppressor, it regulates the expression of genes involved in cell proliferation and differentiation.
Downstream activated genes may include CDKN2A that positively regulates the p53/TP53 pathway, which in turn, prevents cell proliferation.
In spermatogenesis, it probably regulates histone hyperacetylation and the replacement of histones by transition proteins in chromatin, a crucial step in the condensation of spermatid chromatin and the production of functional spermatozoa.
Applications:Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human CHD5.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa1600-1699 from human chromodomain-helicase-DNA-binding protein 5 protein.
