Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation (PubMed:12054757, PubMed:12737517).
Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation (PubMed:17785205, PubMed:20228063).
Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint (PubMed:15790566, PubMed:19223857).
Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1cell cycle arrest and inhibition of cell proliferation (PubMed:16173922).
May play a role in the unfolded protein response, mediating the ufmylation of multiple proteins in response to endoplasmic reticulum stress (PubMed:23152784).
May also play a role in the rupture of the nuclear envelope during apoptosis (PubMed:23478299).
May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1 (PubMed:21283629).
Applications:Suitable for use in ELISA, Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol
Specificity：Recognizes human CDK5RAP3. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu Rt
Immunogen：Recombinant protein corresponding to aa42-292 of human CDK5RAP3. Subcellular Location: Nucleus, Cytoplasm, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
