84-5756-91　CDK2 (FITC) 50ug　558127

特徴

• Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.
• Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2.
• Triggers duplication of centrosomes and DNA.
• Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus.
• Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs).
• Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase.
• EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing.
• Phosphorylates CABLES1 (By similarity).
• Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC.
• Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis.
• In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation.
• Phosphorylation of RB1 disturbs its interaction with E2F1.
• NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication.
• Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase.
• Required for vitamin D-mediated growth inhibition by being itself inactivated.
• Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner.
• USP37 is activated by phosphorylation and thus triggers G1-S transition.
• CTNNB1 phosphorylation regulates insulin internalization.
• Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity).
• Phosphorylates CDK2AP2.
• Applications:Suitable for use in FLISA.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.