84-5744-22　CBL (HRP) 100ug　557498

特徴

• Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors.
• Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome.
• Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling.
• Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation.
• Participates in signal transduction in hematopoietic cells.
• Plays an important role in the regulation of osteoblast differentiation and apoptosis.
• Essential for osteoclastic bone resorption.
• The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function.
• May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.