The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle.
One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity.
The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE.
Required for kinetochore localization of CENPE.
Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification.
Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest.
May play a role for tumor suppression.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human BUB1B.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa230-394 from human mitotic checkpoint serine/threonine-protein kinase BUB1 beta protein.
