The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle.
One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity.
The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE.
Required for kinetochore localization of CENPE.
Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification.
Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest.
May play a role for tumor suppression.
Applications:Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:200Immunoflourescence: 1:50-1:200Immunoprecipitation: 1:200-1:2000Optimal dilutions to be determined by the researcher
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human BUB1B.
Isotype：IgG
Calc Applications Abbrev：E IF IHC IP
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa230-39 fromhuman mitotic checkpoint serine/threonine-protein kinase BUB1 beta protein.
