Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3ubiquitin-protein ligase complex which mediates the ubiquitination andsubsequent proteasomal degradation of target proteins.
Recognizes and binds to phosphorylated target proteins.
SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling.
SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription.
Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'.
SCF(BTRC) mediates the ubiquitination of CEP68; this is required for centriole separation during mitosis (PubMed:25704143, PubMed:25503564).
SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105subunit, ATF4, CDC25A, DLG1, FBXO5, PER1, SMAD3, SMAD4, SNAI1 andprobably NFKB2.
Has an essential role in the control of the clock-dependent transcription via degradation of phosphorylated PER1 and PER2.
May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase.
Required for activation of NFKBmediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF.
Required for proteolytic processing of GLI3.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human BTRC.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa397-448 from human F-box/WD repeat-containing protein 1A protein.
