Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells.
Regulates cell death by controlling the mitochondrial membrane permeability.
Appears to function in a feedback loop system with caspases.
Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release of cytochrome c from the mitochondria and/or by binding to the apoptosisactivating factor (APAF-1).
May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release(PubMed:17418785).
Applications:Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human BCL2.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa31-80 from human apoptosis regulator Bcl-2 protein.
