Component of the beta-catenin destruction complex required for regulatingCTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling.
Controls dorsoventral patterning via two opposing effects; downregulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway.
In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B.
Likely to function as a tumor suppressor.
Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation.
Enhances TGFbeta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7.
Also component of the AXIN1-HIPK2-TP53complex which controls cell growth, apoptosis and development.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human AXIN1.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa418-707 from human Axin-1 protein.
