Ubiquitin-like modifier involved in autophagosomes formation.
With ATG4, mediates the delivery of the autophagosomes to the vacuole via the microtubule cytoskeleton.
Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.
Participates also in membrane fusion events that take place in the early secretory pathway.
Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress.
The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy.
Applications:Suitable for use in FLISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：Candida glabrata
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes Candida glabrata ATG8.
Isotype：IgG
Calc Applications Abbrev：FLISA
Immunogen：Recombinant protein corresponding to aa1-116 from candida glabrata autophagy-related protein 8.
