Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy.
Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity).
Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine.
Exposure of the glycine at the C-terminal is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy.
Has also an activity of delipidating enzyme for the PE-conjugated forms.
Applications:Suitable for use in ELISA, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human ATG4C.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa419-448 from human cysteine protease ATG4C protein.
