Isoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized.
The channel generates a biphasic current with a fast inactivating and a slow sustained phase.
Has high selectivity for sodium ions and can also transport lithium ions with high efficiency.
Isoform 2 can also transport potassium, but with lower efficiency.
It is nearly impermeable to the larger rubidium and cesium ions.
Isoform 3 can also transport calcium ions.
Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis.
This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury.
Heteromeric channel assembly seems to modulate channel properties.
Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines.
Modulates activity in the circuits underlying innate fear.
Applications:Suitable for use in ELISA, Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:5000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Specificity：Recognizes human ASIC1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to aa195-297 from human acid-sensing ion channel 1 protein.
