Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis.
Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins).
Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration.
Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9.
Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes.
Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1.
Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling.
Phosphorylation of CBL leads to increased EGFR stability.
Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components.
ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death.
In response to oxidative stress, phosphorylates serine/threonine kinase PRKD2 at 'Tyr-717'
Applications:Suitable for use in Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:50-1:200ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ABL1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant Protein corresponding to human ABL1.
