SARS-CoV-2 is part of the Coronaviridae family, subfamily Orthocoronavirinaes and includes the genera Alphacoronvirus, Betacoronavirus, Gammacoronavirus, and Deltacoronavirus.
Alphacoronaviruses and betacoronaviruses typically infect mammals, whereas gammacoronoviruses and deltacoronaviruses generally infect avian species (and sometimes mammals).
Two types of alphacoronaviruses (229E and NL63) and two types of betacoronaviruses (OC43 and HKU1) are pathogens of humans and cause the common cold.
Whereas, SARS-CoV-1, the Middle Eastern respiratory syndrome coronavirus (MERS-CoV), and SARS-CoV-2 (all betacoronaviruses) cause more severe disease in humans including, respiratory, gastrointestinal, hepatic, and neurologic diseases (Amanat & Krammer, 2020).
SARS-CoV-2 has a large single-stranded positive-sense RNA genome encoding for several open reading frames.
One frame encodes the spike protein (S protein), a class I fusion protein that forms homotrimers protruding from the viral surface and mediates coronaviruses attachment and adhesion to human target cells via a host receptor.
Spike has two different functional regions, S1 and S2.
The S1 subunit consists of four core domains, S1A to S1D.
The distal S1 domain mediates receptor association and stabilization, whereas the S2 domain promotes structural rearrangements and membrane fusion.
Coronaviruses use different regions of S1 domain to interact with specific binding receptors.
For SARS-CoV-2, the receptor binding domain (RBD) interacts with the peptidase domain of the angiotensin-converting enzyme 2 (ACE2) receptor from bats, civet cats, swine, cats, ferrets, non-human primates (NHPs), and humans (Amanat & Krammer, 2020).
ACE2 is a membrane-anchored carboxypeptidase highly expressed by airway epithelial and type I and II alveolar epithelial cells (Perrotta et al., 2020).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000ELISA: 1:4000-1:14,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography from culture supernatant.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes the SARS-CoV-2 Spike glycoprotein, subunit 2 (S2). Does not cross-react in ELISA with HCoV-229E full-length spike protein
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Recombinant protein corresponding to aa685-1211 from SARS-CoV-2 Spike (S2), fused to His-Tag at C-terminal, expressed in insect cells.
