X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of misfolded proteins in the endoplasmic reticulum (ER).
XBP-1 mRNA is spliced by IRE1p under ER stress, resulting in a 371aa protein (XBP- 1s) which is translocated to the nucleus where it binds to the regulatory elements of downstream genes.
XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones grp78 and grp94.
Applications:Suitable for use in Western Blot and Immunocytochemistry and Flow Cytometry.
Other applications not tested.
Recommended Dilution:Immunocytochemistry: 1:100Flow Cytometry: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human XBP-1. Species Crossreactivity: sheep
Isotype：IgG
Calc Applications Abbrev：FC IC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human XBP-1, spliced isoform XBP1(S) at C-terminal.
