Wilms tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system.
The gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilms tumor and mesothelioma.
WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor.
Lung adenocarcinomas rarely stain positive with this antibody.
WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
The WT1 recombinant monoclonal antibody can be used to qualitatively identify the presence of WT1 protein in FFPE tissue sections using IHC test methods
For IHC applications: Dilute the concentrate as appropriate in antibody diluent, and then apply 150 µL to the slide, as indicated in the user's IHC protocol.
Applications:Suitable for use in Immunohistochemistry, Flow Cytometry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 0.5-1ug/mlImmunohistochemistry (formalin-fixed paraffin sections): 1:100-1:1000 or 0.5-1ug/ml for 30 min at RT)Flow Cytometry: 0.5-1ug/106 cells in 0.1mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 1X PBS, 0.1mg/ml BSA, 0.05% sodium azide.
Specificity：Recognizes human WT1.
Clone # USB：WT1/1434R
Isotype：IgG,k
Calc Applications Abbrev：FC IF IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to full length human WT1.
