Epitope tagging is a widely accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression.
With this technique, gene expression can be easily monitored on Western blotting, immunoprecipitation and immunofluorescence utilizing an antibody that recognizes such an epitope.
The VSV-G Tag (YTDIEMNRLGK) corresponds to the partial peptide sequence of the vesicular stomatitis virus glycoprotein.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:100, ColorimetricImmunocytochemistry: 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Purified
Purity：Purified by ammonium sulfate precipitation from ascites.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes VSV-G Epitope Tag. Species Crossreactivity: human, mouse and rat
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to Vesicular stomatitis virus (VSV-G) tag (YTDIEMNRLGK) coupled to GST.
