84-5671-97　［取扱停止］VASP 100ul　378659

特徴

• VASP (vasodilator stimulated phosphoprotein) is a proline-rich protein substrate of cAMP and cGMP-dependent protein kinases.
• Phosphorylation of VASP at Ser-157 causes a mobility shift in SDS gel electrophoresis from 46-50kD, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity.
• VASP is the founding member of the Ena-VASP protein family, comprising the Drosophila protein Enabled (Ena), its mouse homologue Mena (mammalian Enabled), and mouse EVL (Ena-VASP-like protein).
• With these proteins VASP shares a conserved overall domain organization: a) the conserved N-terminal Ena-VASP homology domain 1 (EVH1), which mediates binding to a proline-rich motif b) a more divergent proline-rich central domain (which is responsible for profilin binding) c) a conserved C-terminal EVH2 domain
• VASP is expressed in a variety of mammalian cell types and tissues.
• In cultured cells, VASP is associated with focal adhesions, cell-cell contacts, microfilaments, and highly dynamic membrane regions.
• From in vitro binding data VASP has been suggested to link profilin to zyxin, vinculin, and the Listeria spp.
• surface protein ActA, respectively.
• Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation and the actin-dependent motility of intracellular bacterial pathogens.
• Applications:Suitable for use in Western Blot and Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1500-1:3000Immunofluorescence: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.