The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress.
It includes cytoplasmic (Hsp90-alpha/beta), ER (Grp94) and mitochondrial (TRAP1) localized members.
Hsp90 is structurally composed of an N-terminal ATP binding domain, a medial substrate-binding region, and a C-terminal dimerization motif.
Hsp90 dimers function in cooperation with cochaperones to stabilize a multitude of client protein substrates.
TRAP1 (aka Hsp75) was originally identified as a chaperone binding partner for retinoblastoma protein.
TRAP1 was later found by immunofluorescence to be localized to the mitochondria.
TRAP1 is significantly more active as an ATPase due in part to the lack of regulatory elements in the C-terminus, but retains the ability to dimerize.
However, it does not form stable complexes with typical cochaperones of Hsp90 like p23 and HOP4.
TRAP1 has been identified as a protective element for cell survival, and has been suggested as a target for anti-cancer therapeutics.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, ColorimetricOptimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human TRAP1. Species Crossreactivity: human, mouse, rat and bovine
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Recombinant protein corresponding to human TRAP1.
