84-5671-86　［取扱停止］Tau, phosphorylated (Tyr18) 100ul　378607

特徴

• Tau is the principal microtubule-associated protein located in axons in the nervous system where it participates in events including the elongation, stabilisation and bundling of microtubules.
• The tau family consists of six isoforms identified by the insertion of three or four partial repeats of aa30-31 in the C-terminal region.
• Subcellular fractionation studies have revealed tau to be associated with the membrane fraction, as well as with the cytoskeletal fraction and there is some evidence that the N-terminal domain of tau may be responsible for membrane binding as well as interaction with other cellular components.
• Tau extracted from human fetal and neonatal brains is moderately phosphorylated with 5-9 phosphorylated serine/threonine residues per molecule.
• Normal adult human brain tau shows a marked reduction in phosphorylation, but in certain neurodegenerative diseases (tauopathies) tau becomes hyperphosphorylated (6-15 phosphates per tau molecule have been identified) and associates to form filamentous aggregates.
• In Alzheimer’s disease (AD) hyperphosphorylated tau polymerises to form a mixture of paired helical filaments (PHF) and straight filaments which accumulate and contribute to the formation of neurofibrillary tangles - one of the characteristic features of AD.
• It was reported in 1998 that tau could be tyrosine phosphorylated when co-transfected with the src-family protein tyrosine kinase fyn.
• In 2004 the same group, using phospho-specific antibodies, reported that fyn phosphorylated Tyr18 of brain tau at an early developmental stage in mice, but that tyrosine phosphorylated tau was undetectable in adult mice.
• However, they also showed that PHF preparations were reactive to the tau-pTyr18-specific antibodies, implicating a role for fyn – and possibly other tyrosine kinases - in the neurodegenerative process.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:3000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.