84-5671-84 ［取扱停止］SIRT5 100ul 378593
特徴
- SIRT5 is the only human class III sirtuin and as such is the closest homolog to bacterial (e.g.
- E.
- coli CobB) and archaeal sirtuins.
- SIRT5 is an imported mitochondrial protein with a calculated precursor molecular weight of 34kD.
- In various human, rat, mouse and bovine cells and tissues, the major reactive band migrates with an apparent molecular weight of ~29kD.
- This presumably reflects removal of an N-terminal transit sequence due to mitochondrial import and processing.
- No cross-reactivity observed with recombinant human sirtuins SIRT1, SIRT2 or SIRT3.
- Applications:Suitable for use in Western Blot.
- Other applications not tested.
- Recommended Dilution:Western Blot: 1:2500, Colorimetric, using secondary antibody coupled to alkaline phosphatase and BCIP/NBT as substrate.
- / 1:3000, ECL, using secondary antibody coupled HRP)Optimal dilutions to be determined by the researcher.
- Storage and Stability:May be stored at 4°C for short-term only.
- Aliquot to avoid repeated freezing and thawing.
- Store at -20°C.
- Aliquots are stable for 12 months after receipt.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
- Size：100ul
- Host：rabbit
- Source Antibody：human
- Grade：Purified
- Purity：Partially purified from rabbit serum with immunodepletion.
- Form：Supplied as a liquid in PBS.
- Specificity：Recognizes human SIRT5. Species Crossreactivity: mouse and rat
- Isotype：IgG
- Calc Applications Abbrev：WB
- Calc Crossreactivity：Bo Hu Mo Rt
- Immunogen：Recombinant protein corresponding to human SIRT5.
- Shelf Life：1year
- Swiss Prot Number：Q9NXA8
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