There are 3 alternatively spliced isoforms of the Shc adapter protein: p46SHC, p52SHC and p66SHC, that link activated growth factor receptors to signalling pathway.
Isoform p46SHC and p52SHC, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS com-plex and are implicated in mitogenic signalling.
They function as initiators of the Ras signalling cascade in various non-neuronal systems.
The isoform p66SHC does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span.
It acts as a downstream target of the tumor suppressor p53 and is indispen-sable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes human p46shc, p52shc and p66shc isoforms of phosphorylated and unphosphorylated Shc.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hm Hu Mo
Immunogen：Recombinant protein corresponding to aa1180-1620 from human Shc.
