PKA (protein kinase A), also known as cAMP-dependent protein kinase, phosphorylates serine or threonine residues in target protein in response to elevated levels of cAMP.
Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits.
Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, ColorimetricOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human PKA. Species Crossreactivity: human, mouse, rat, bovine, canine, guinea pig, haster, monkey, porcine, sheep, xenopus.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Ca Gp Hm Hu Mk Mo Po Rb Rt Sh Xe
Immunogen：Synthetic peptide corresponding to human PKA.
