p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis.
It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein.
Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma.
Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia.
Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlImmunofluorescence: 0.5-1ug/mlImmunohistochemistry (formalin-fixed paraffin sections): 0.25-0.5ug/ml for 30 min at RT)Flow Cytometry: 0.5-1ug/106 cells in 0.1mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 1X PBS, 0.1mg/ml BSA, 0.05% sodium azide.
Specificity：Recognizes he mutant as well as the wild form of p53.
Clone # USB：TP53/1799R
Isotype：IgG,k
Calc Applications Abbrev：FC IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human p53.
