Nuclear factor kappa-B (NFk) B) functions as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes.
NF-kB is a heterodimer consisting of a 50kD DNA binding subunit (p50) and a 65kD transactivation subunit (p65/RelA) (ref.
3), both of which exhibit sequence homology to the protooncogene c-Rel.
p50 has an isoform called p49/p52, and both proteins derive from the N-terminal of precursor protein p105 and p100, respectively.
The p50/p65 heterodimer remains in inactive form in the cytosol as a complex with its inhibitor, IkB.
Upon stimulation of cells by a variety of stimuli, including lipopolysaccharide (LPS), pro-inflammatory cytokines like interleukin-1 and tumor necrosis factor, and viral infection, IkB becomes phosphorylated and degraded by the proteosome.
The active NF-kB heterodimer translocates into the nucleus and induces gene expression.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, ColorimetricOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human NFkappaB p65. Species Crossreactivity: human, mouse, rat, bovine, canine, guinea pig, monkey, porcine and rabbit
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Ca Gp Hu Mk Mo Po Rb Rt
Immunogen：Synthetic peptide corresponding to a portion of human p65 NF-kappaB. The sequence is completely conserved in mouse NF-kappaB p65.
