mTOR (FKBP12-rapamycin associated protein (FRAP)) is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection.
It has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion.
FKBP12-rapamycin-binding (FRB) domain is a conserved 11kD region necessary for FKBP12-rapamycin binding.
FRB is one of several functional domains of mTOR: HEAT (Huntington elongation factor 1A-protein phosphatase 2A-A subunit-TOR); FAT (FRAP, ATM, TTRAP2); PIKK (PI3-kinase-related kinase); RD (regulatory domain); and FATC (FAT, C terminal)
Applications:Suitable for use in Western Blot, Immunocytochemistry and ChiP.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000Optimal dilutions to be determined by the researcher..
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1Vial
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from sodium azide. Reconstitute with 50ul sterile dH2O.
Specificity：Recognizes the FRB domain of fusion proteins expressed in Saccharomyces cerevisiae (see H. Haruki, et al. 2008) and the human FRB domain of mTOR.
Isotype：IgG
Calc Applications Abbrev：ChIP IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human mTOR (FKBP12-rapamycin-binding (FRB) domain).
