MEKKs (Mitogen activated protein kinase kinase kinases) are serine-threonine kinases that act as the first tier of cellular MAP kinase pathways by activation of MAP/ERK kinases, or MEKs.
Many enzymes with MEKK activity have been identified, including MEKK1-4, Raf, MLK3, TAK, and DLK.
MEKKs generally display little similarity outside of their catalytic kinase domains.
MEKK1-4 are nearly 50% identical within their catalytic domains, and are known to regulate Erk, Jnk, and p38 MAP kinase pathways.
MEKK2 and MEKK3 bind MEK5 via conserved PB1 domains, leading to downstream activation of Erk5.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid.
Specificity：Recognizes mouse MeKK3. Species Crossreactivity: human, ratcanine, drosophila, guinea pig, hamster, monkey, porcine, rabbit, sheep and xenopus
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Dr Gp Hm Hu Mk Mo Po Rb Rt Sh Xe Cm
Immunogen：Synthetic peptide corresponding to the sequence of mouse MEKK3 at N-terminal.
