Mcl-1 is a member of the Bcl-2 family that possesses anti-apoptotic activity.
Mcl-1 is an immediate-early gene activated by the GMCSF as well as IL-3 signaling pathways and plays an important role in the viability response of these cytokines.
Similar to all Bcl-2 family members, Mcl-1 contains BH1, BH2, and BH3 domains; however, unlike other members of this family, Mcl-1 has an extended N-terminal domain rich in PEST sequences, which probably accounts for the short half-life of this protein.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000, ECLImmunohistochemistry: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human Mcl-1. Species Crossreactivity: canine, guinea pig, monkey, porcine and rabbit
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Ca Gp Hu Mk Po Rb
Immunogen：Synthetic peptide corresponding to a portion of human Mcl-1.
