Irisin is a hormone that is involved in converting white adipose tissue into brown adipose tissue after physical exercise.
It is formed after the membrane bound protein fibronectin type III domain containing protein 5 (FNDC5) is cleaved resulting in a secreted protein.
FNDC5 is found in muscle tissue and is activated during physical exertion via the protein PGC1a.
Soluble Irisin has also been shown to up regulate UCP-1 gene expression in mice.
UCP-1 is a mitochondrial transporter protein and a marker of brown adipose tissue conversion.
This suggests that Irisin plays an important role in energy regulation and furthermore may lead to improvements in glucose regulation, insulin sensitivity, hypertension, and obesity.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography and immunodepleted.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human Irisin.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Irisin (active).
