The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78kD, and are the eukaryotic equivalents of the bacterial DnaK.
The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78).
Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains.
Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding.
Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, ECLOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol
Specificity：Recognizes human Hsp70. Species Crossreactivity: human, mouse, ratbeluga, bovine, canine, hamster, monkey, porcine and sheep.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Bo Ca Hm Hu Mk Mo Po Rt Sh
Immunogen：Synthetic peptide corresponding to human Hsp70.
