84-5671-34　［取扱停止］HSP25 (PE) 200ug　378403

特徴

• In response to adverse changes in their environment, cells from many organisms increase expression of a class of proteins referred to as heat shock or stress proteins.
• Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30kD.
• Studies on estrogen-induced proteins in breast cancer cells suggest a direct relationship between elevated stress protein expression and neoplasia.
• Hsp27 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g., phorbol esters) and calcium ionophores1 and elevated levels of Hsp27 correlate with endometrial adenocarcinomas.
• Interestingly, the regulation of human Hsp27 by estrogens and heat shock resembles the ecdysteroid and heat shock activation of the drosophila Hsp20 genes.
• This antibody reacts with both the phosphorylated and the non-phosphorylated forms of Hsp25.
• Applications:Suitable for use in Flow Cytometry.
• Other applications not tested.
• Recommended Dilution:Flow Cytometry 1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Further dilutions can be made in assay buffer.
• Caution: PE conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.