The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin.
The core histones form an octamer, which is made up of one H3-H4 tetramer and two H2A-H2B dimers.
The N-terminal tail of core histones undergoes different post-transcriptional modifications including acetylation, phosphorylation, methylation and ubiquitination.
These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human Histone H3. Species Crossreactivity: mouse, rat, chicken, Drosophila, monkey, porcine and rabbit
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Ch Dr Hu Mk Mo Po Rb Rt
Immunogen：Synthetic peptide corresponding to human Histone H3 at Ser10.
