FLAG-tag stands for an octapeptide with a sequence of DYKDDDDK.
It is a polypeptide protein tag that can be added to a protein using recombinant DNA technology.
It is widely used for affinity chromatography to purify recombinant proteins from variety of systems.
It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody.
If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence, such as in ELISA, Western Blot, etc.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
torage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes FLAG-tagged proteins.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Synthetic peptide corresponding to CDYKDDDDK, conjugated to (KLH).
