Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GISTs), c-kit signaling-driven mesenchymal tumors of the GI tract.
DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose.
Immunoreactivity for DOG-1 has been reported in 97.8 % of scorable GISTs, including all c-kit negative GISTs.
Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including platelet-derived growth factor Receptor alpha mutants that fail to express c-kit antigen.
The overall sensitivity of DOG1 and c-kit in GISTs is nearly identical: 94.4% vs.
94.7%.
Applications:Suitable for use in Immunohistochemistry, Flow Cytometry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 0.5-1ug/106 cells in 0.1 mlImmunofluorescence: 0.5-1ug/mlImmunohistochemistry (formalin-fixed paraffin sections): 0.25-0.5ug/ml for 30 min at RTOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 1X PBS, 0.1mg/ml BSA, 0.05% sodium azide.
Specificity：Recognizes human DOG1.
Clone # USB：DG1/1487R
Isotype：IgG,k
Calc Applications Abbrev：FC IF IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to full length human DOG1.
