Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata.
DIG is the steroid component of digoxin, a cardiac glycoside, and can be conjugated onto a variety of biomolecules (proteins, nucleic acids) to be detected in further assays (e.g.
ELISA).
These antibodies can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection.
DIG has an advantage over biotin due to the fact that biotin is an endogenous molecule of living cells and plays a key role in many reactions, which can sometimes lead to false positive results.
Because the blossoms and leaves of Digitalis plants are the only natural sources of digoxigenin, binding of the anti-digoxigenin antibody in other biological material is not a consideration.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：sheep
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
