CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway.
In addition, it may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma.
Cyclophilin A binds to CD99 and may act as a signaling regulator of the protein.
Applications:Suitable for use in Immunohistochemistry, Flow Cytometry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 2-4ug/mlImmunohistochemistry (formalin-fixed paraffin sections): 5-10ug/ml for 30 min at RT)Flow Cytometry: 2-4ug/106 cells in 0.1mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 1X PBS, 0.1mg/ml BSA, 0.05% sodium azide.
Specificity：Recognizes human CD99.
Clone # USB：MIC2/1495R
Isotype：IgG,k
Calc Applications Abbrev：FC IF IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to full length human CD99.
