CD51 (integrin alpha V subunit) associates with CD61 (integrin beta 3 subunit) binding to the RGD sequence in extracellular matrix proteins like vitronectin, fibrinogen, thrombospondin, von Willebrand factor and osteopontin.
The alphaV/beta3 heterodimer is expressed in endothelial cells, leukocytes, NK cells, macrophages, neutrophils, platelets and several malignant tumor cells and seems to play a role in embryo implantation, angiogenesis, wound healing and cancer.
alpha V/beta 3 is also highly expressed in osteoclasts being important for bone resorption.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:5000Immunohistochemistry (frozen): 1:500Immunohistochemistry: (paraffin) 1:500Immunocytochemistry: 1:1000Immunoprecipitation: 5ul per 5x10e6 cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes the cytoplasmic domain of CD51.
Isotype：IgG
Calc Applications Abbrev：E IC IHC IP WB
Immunogen：Synthetic peptide corresponding to aa1021-1034 of human CD51 (alpha V integrin) at C-terminal.
