The caspases are a family of cysteine proteases that cleave after certain aspartate residues, and are primarily recognized as mediators of apoptosis.
caspases are synthesized as inactive zymogens that can be cleaved to form active enzymes following the induction of apoptosis by stress or death receptors.
Initiator caspases (e.g.
caspase-8 and -10) are activated by dimerization of the zymogen on a dedicated adaptor protein.
These activated initiator caspases in-turn cleave downstream effector or ?executioner? caspases (e.g.
caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:5000, ECLOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid.
Specificity：Recognizes human Caspase-3. Species Crossreactivity: bovine, canine, chicken, guinea pig, hamster, monkey, mouse, porcine, rabbit, rat, sheep and xenopus.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Ca Ch Gp Hm Hu Mk Mo Po Rb Rt Sh Xe
Immunogen：Synthetic peptide corresponding to a portion of human caspase-3 . The sequence differs from rat caspase-3 by 1aa and from mouse by 2aa.
