Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies.
The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kD.
Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlFlow Cytometry: 0.5-1ug/106 cells in 0.1 mlImmunofluorescence: 1-2ug/mlImmunohistochemistry (formalin-fixed paraffin sections): 0.25-0.5ug/ml for 30 min at RTOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 1X PBS, 0.1mg/ml BSA, 0.05% sodium azide.
Specificity：Recognizes basic (Type II or HMW) cytokeratins, which include 67kD (CK1); 64kD (CK3); 59kD (CK4); 58kD (CK5); 56kD (CK6); 52kD (CK8)
Clone # USB：KRTH/1576R
Isotype：IgG,k
Calc Applications Abbrev：FC IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to full length human kRT76.
