Aurora A, B, and C have emerged as key mitotic regulators of mammalian centrosome function, chromosome segregation and cytokinesis.
Aurora B is one of several chromosomal passenger proteins involved in chromosome alignment at the metaphase plate and cytokinesis.
Phosphorylation of a threonine residue in the Aurora activation loop (Thr232 in human Aurora B) is believed to be an essential regulatory mechanism in Aurora kinase activation.
Applications:Suitable for use in Western Blot and immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml, ECL
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human Aurora B Thr232.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to a portion of human Aurora B phosphorylated at Thr232.
