The octapeptide circulatory hormone Angiotensin II is a critical regulator of cardiovascular homeostasis formed in blood, with dual function in tissues of the brain, kidney, heart, and vasculature as an autocrine/paracrine growth hormone.
The actions of angiotensin II are mediated by type one (AT1) and type two (AT2) angiotensin II receptors, members of the seven-transmembrane Gprotein coupled receptor (GPCR) family.
AT1 receptors couple predominantly with Gq/11 alpha subunits, and signal through phospholipases C, D, and A2, inositol phosphates, and calcium channels, as well as a variety of serine/threonine and tyrosine kinases.
AT1 expression is greatest in vascular smooth muscle, liver, kidney, adrenal cortex, brain, and lung and is primarily responsible for the physiological effects of angiotensin II, including regulation of arterial blood pressure, electrolyte and water balance, thirst, hormone secretion and renal function.
The AT2 receptor displays approximately 30% identity to the AT1 receptor, and is highly expressed during embryonic development.
AT2 expression in adult tissue is limited to the adrenal medulla, brain, and reproductive tissues, but is upregulated in injured tissue following myocardial infarction, vascular injury, and wound healing.
AT2 receptor signaling is not dependent on G-protein coupling or receptor phosphorylation, although coupling to Gia2 and Gia3 alpha subunits occurs in some tissues.
AT2 activation can stimulate tyrosine and serine/threonine phosphatases through activation of nitric oxide and cGMP, and appears to counteract the stimulatory effects of AT1 receptor and growth factor receptor signaling.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.01% sodium azide,
Specificity：Recognizes rat Angiotensin II receptor AT2.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to rat angiotensin II receptor AT2 at N-terminal. Species sequence homology: mouse and gerbil
