84-5670-51　［取扱停止］Akt 200ug　378062

特徴

• The Akt (PKB) family of protein kinases are serine/threonine kinases, with three mammalian family members identified (Akt1, Akt2, Akt3).
• Akt is a well-characterized member of PI3 kinase-mediated signaling pathways, regulating cell growth, apoptosis, glycogen synthesis, and other cellular responses through its phosphorylation of downstream substrates.
• Akt activation is triggered by binding of phospholipid and phosphorylation at two key residues: Thr308 by PDK1, and Ser473 by PDK2, now identified as mTOR.
• Deregulation of Akt signaling has been associated with cancer, diabetes, and schizophrenia.
• Akt1 is the cellular homologue of the murine thymoma retroviral oncogene v-akt, and its role in anti-apoptotic and pro-mitotic pathways have made Akt a molecular target for anti-cancer therapeutic intervention.
• Akt activation inhibits apoptosis by phosphorylating the Bcl-2 related protein Bad, and increases p53 degradation by phosphorylating mdm2.
• Mitotic substrates of Akt include GSK-3beta, p21CIP1, and p27KIP1, cell cycle inhibitors negatively regulated by Akt phosphorylation.
• Akt has been shown to mediate angiogenesis through regulation of thrombospondins, which may cooperate with pro-mitotic and anti-apoptotic functions of Akt to promote tumorigenesis.
• Applications:Suitable for use in Western Blot, Immunoprecipitation and immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000, colorimetricOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.