Actin is one of the major proteins (~42kD) of the cytoskeleton.
It regulates contractile potential in muscle tissues and helps to control the shape as well as the motility of non-muscle cells.
Actin is expressed in all eukaryotic cells which makes it a useful loading control in Western blotting experiments.
In higher eukaryotes, there are six isoforms of actin: Three alpha-actins (alpha-skeletal, alpha-cardiac, alpha-smooth muscle), one beta-actin (beta-non-muscle) and two gamma-actins (gamma-smooth muscle and gamma-non-muscle).
Actin isoforms show >90% overall sequence homology.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, ECLOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes bovine Actin. Species Crossreactivity: human, mouse, rat, bovine, chicken, canine, guinea pig, hamster, monkey, porcine, rabbit, sheep and xenopus.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Ca Ch Gp Hm Hu Mk Mo Po Rb Rt Sh Xe
