84-5670-45　［取扱停止］Acidic Cytokeratin 100ug　378054

特徴

• There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins.
• The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development.
• Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile.
• Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation.
• The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-1ug/mlImmunofluorescence: 1-2ug/mlImmunohistochemistry (formalin-fixed paraffin sections): 1-2ug/ml for 30 min at RTFlow Cytometry: 0.5-1ug/106 cells in 0.1mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.