Siah-1 is a member of the seven in asentia homolog (SIAH) family.
It is a E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, including many proteins involved in transcription regulation, a cell surface receptor (DCC), cytoplasmic signal transduction molecules, an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP) and a structural protein.
Consequetly Siah-1 is involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon gyidance, transcription regulation, spermatogenesis, and TNF-alpha signaling.
It is predominantly a cytoplasmic protein but is also partialy a nuclear protein.
It is widely expressed at low levels and is down-regulated in advanced hepatocellular carcinomas.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：mouse
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 50% glycerol.
Specificity：Recognizes human Shiah-1.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Partial recombinant protein corresponding to aa1-110 from human Shiah-1, fused to GST-Tag.
