Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by ammonium sulfate precipitation followed by affinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human CD74.
Clone # USB：SearchType in product code or name:Goat Anti-CD74 (aa159-171) Antibody (EB12551)Related Products: EBP12551 - Immunizing Peptide, EBP12552 - Immunizing Peptide, Goat Anti-CD74 AntibodyCodeNameApplicationsAvailabilitySizeTested speciesPriceGrade EB12551Goat Anti-CD74 (aa159-171) AntibodyPep-ELISA, WB, IHC100µg specific antibody in 200µlHuman$324.00Ordering Information for United States of America (change)Order direct from Everest$49.00: Overnight shipping (not applicable to SB-shifix) to United States of AmericaSent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.Order offlineOROrder from our local distributorARP American Research Products, IncView contact detailsProduct detailsReviewsProtocols and MSDSEB12551 (0.01µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.EB12551 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.Click here to download PDF datasheet.Target ProteinPrincipal Names: CD74, DHLAG, HLADG, Ia-GAMMA, CD74 molecule, major histocompatibility complex, class II invariant chain, CD74 antigen, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), HLA-DR-gamma, Ia-associated invariant chain, MHC HLA-DR gamma chain, gamma chain of class II antigensOfficial Symbol: CD74Accession Number(s): NP_001020330.1; NP_004346.1Human GeneID(s): 972 Important Comments: This antibody is expected to recognize isoform a (NP_001020330.1) and isoform b (NP_004346.1).ImmunogenPeptide with sequence C-KGSFPENLRHLKN, from the internal region of the protein sequence according to NP_001020330.1; NP_004346.1.Please note the peptide is available for sale.Purification and StoragePurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:128000.Western blot: Approx 33kDa band observed in Human Tonsil lysates (calculated MW of 33.5kDa according to NP_001020330.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.Species ReactivityTested: HumanExpected from sequence similarity: Human
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa159-171, C-KGSFPENLRHLKN, from human CD74 from the internal region.
