The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues.
Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins.
Purified GFP is a 27kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509nm) when excited with blue or UV light.
Applications:Suitable for use in Immunocytochemistry, Western Blot and ELISA.
Other applications not tested.
Recommended Dilutions:ELISA: 1:800-1:1000Western Blot: 1:500-1:1000 using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods
Optimal dilutions to be determined by the researcher.
Control:Cells transfected with GFP-tagged fusion vectorRENCX cells
Note:While this antibody has been adsorbed against E.
coli proteins, most secondary antibodies have not.
To reduce the likelihood of E.
coli reactivity from a secondary antibody, use highly-purified secondary antibodies in indirect detection systems.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a liquid in 10mM Tris buffer, pH 8.0, 0.01% sodium azide
Specificity：Recognizes GFP from both native and recombinant sources. Minimal crossreactivity with E. coli.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Immunogen：Highly purified native GFP from Aequorea victoria. Species Sequence Homology: vertebrates and rat
