Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle.
Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues, suggesting a post-transcriptional regulation of cyclin E.
In vitro analysis indicated that these truncated variant isoforms of cyclin E are able to phosphorylate histone H1.
Alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 10min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20minImmunoprecipitation: 10ug/mlWestern Blot: 5-10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Specificity：Recognizes human Cyclin E. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IF IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to the C-terminus of human Cyclin E.
