The mRNA Cap-binding protein eIF4E is involved in an early rate limiting step in the initiation of protein synthesis.
In response to growth factors and other extracellular stimuli, eIF4E is phosphorylated at Ser209.
This phosphorylation increases the affinity of eIF4E to capped-mRNAs.
The Mitogen-Activated Protein Kinase Interacting Kinases (Mnk’s) and an insulin-stimulated cytosolic Protamine Kinase (cPK) phosphorylate eIF4E at Ser209 in cells.
In vitro, cPK also phosphorylates eIF4E at Thr210.
eIF4E occurs in cells in an inactive form as a complex with inhibitory binding proteins (4EBP).
It also occurs in an active form as part of the eIF4F complex which also contains eIF4A and eIF4G.
In response to insulin and other extracellular stimuli, eIF4E is recruited to the eIF4F complex following its dissociation from 4EBP.
Over-expression of eIF4E leads to cell transformation and tumorigenesis.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500 with 50ug of bovine liver extracts
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid.
Specificity：Recognizes human eIF4E. Crossreacts with eIF4E from diverse tissue sources.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human eIF4E.
