84-5614-91　［取扱停止］BRAF V600-E mutation (Concentrated) 100ul　544711

特徴

• BRAF is a major component in the intracellular signalling of several important membrane tyrosine kinases, having a powerful activating potential on the MAP kinase dependent pathways.
• A point mutation specified by the V600-E amino acid exchange results in the transactivation of the pathway and is a major driver of oncogenesis.
• The BRAF V600-E mutation and expression of the mutant protein is therefore of great clinical importance in many malignancies.
• BRAF (V600-E) mutations are found in approximately 10% of colorectal cancers(1) and 50% of melanomas (2).
• They are also linked to cancers such as papillary thyroid cancer and hairy cell leukemia.
• Thus, antibodies against the V600-E BRAF mutant protein have the potential to perform a vital role in the immunohistochemical analysis of tumor samples.
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:200Optimal dilutions to be determined by the researcher.
• Positive Control: Colon carcinoma tissue, melanoma tissue
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.